5 edition of Single-Molecule Detection in Solution Methods and Applications found in the catalog.
January 15, 2002
Written in English
|Contributions||Christoph Zander (Editor), Jörg Enderlein (Editor), Richard A. Keller (Editor)|
|The Physical Object|
|Number of Pages||400|
Recently, new methods have been developed which although not being explicitly 'single-molecule' are approaching exceptional levels of detection precision in requiring only nanocrystals while keeping molecular damage by x-rays at an acceptable level. Nanocrystals of the target protein are fired in solution through a femtosecond x-ray beam and Cited by: Single-Molecule Optical Detection, Imaging and Spectroscopy W. E. Moerner, M. Orrit, U. P. Wild, Thomas Basché Presents the latest developments in detecting and probing single entities.
Single molecule detection techniques are also essential in overcoming the diffraction limit in microscopy and have been honored with the Nobel prize in chemistry. PicoQuant has a long history of supporting the single molecule community both with state of the art instrumentation as well as through hosting single molecule events around the. Single molecule detection methods, such as nanopore sensors have found increasing importance in applications ranging from gaining a better understanding of biophysical processes to technology driven solutions such as DNA sequencing. However, challenges remain especially in relation to improving selectivity t Most Impactful Nanoscience Articles.
A single-molecule experiment is an experiment that investigates the properties of individual -molecule studies may be contrasted with measurements on an ensemble or bulk collection of molecules, where the individual behavior of molecules cannot be distinguished, and only average characteristics can be measured. Since many measurement techniques in . Ha, T., “Single-Molecule Fluorescence Resonance Energy Transfer” Methods. (). Xu et al., “Direct Measurement of Single-Molecule Diffusions and Photodecomposition in Free Solution” Science, (Feb. 21, ). Casoli et al., “Fluorescence Correlation Spectroscopy as a Tool to.
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This handbook provides a thorough overview of the field. It begins with basics of single molecule detection in solution, describes methods and devices (fluorescense correlation spectroscopy, surface enhanced Raman scattering, sensors, especially dyes, screening techniques, especially confocal laser scanning microscopy).Format: Hardcover.
About this book. The detection of single molecules opens up new horizons in analytical chemistry, biology and medicine. This discipline, which belongs to the expanding field of nanoscience, has been rapidly emerging over the last ten years. This handbook provides a thorough overview of the field.
This handbook provides a thorough overview of the field. It begins with basics of single molecule detection in solution, describes methods and devices (fluorescense correlation spectroscopy, surface enhanced Raman scattering, sensors, especially dyes, screening techniques, especially confocal laser scanning microscopy).
With contributions from leading authors in the field, this book provides an excellent overview (with references) on the principles, techniques, and potential applications of single-molecule detection.
It is a must-read for anyone who is interested in applying single-molecule techniques to their own research.5/5(1).
Get this from a library. Single molecule detection in solution: methods and applications. [Ch Zander; J Enderlein; Richard A Keller;] -- "The detection of single molecules opens up new horizons in analytical chemistry, biology and medicine.
This discipline, which belongs to the expanding field of nanoscience, has been rapidly emerging. The detection of small concentration is extremely important in fundamental research and various applications.
The ability to detect a single atom or molecule is a key aspect of investigating single quantum object properties and QED effects . These temporal fluctuations, which are typical signature of single molecule detection, were attributed to the changes of molecular orientations of the sample molecules Author: Jerker Widengren.
However, formatting rules can vary widely between applications and fields of interest or study. The specific requirements or preferences of your reviewing publisher, classroom teacher, institution or organization should be applied.
Surface-enhanced Raman Scattering (SERS) - A Tool for Single Molecule Detection in Solution. Single Molecule Detection on Surfaces with the Confocal Laser Scanning Microscope. Spectroscopy of Individual Photosynthetic Pigment-Protein Complexes. Single-Molecule Identification in Solution: Principles and Applications.
Abstract. Single-molecule methods have emerged as powerful tools in life science research. These techniques allow the detection and manipulation of individual biological molecules and investigate, with unprecedented resolution, their conformations and dynamics at the nanoscale by: 2.
Detection of single molecules represents the ultimate level of sensitivity and has been a longstanding goal of analytical methods. Because of its high sensitivity, and because a bright signal appears against a dark background, fluorescence is one obvious choice for single-molecule detection (SMD).
Single Molecule Detection, Analysis, and Manipulation Nils G. Walter University of Michigan, Ann Arbor, USA 1 Introduction 1 2 History and Breadth of Applications 1 3 Preparation and Handling of the FEW 2 Preparation of Single Fluorescent Molecules or Particles 3 Immobilization or Trapping of Single Molecules for Fluorescence Detection 3.
Optical Methods for Single Molecule Detection and Analysis. As analytical chemists, the highest resolution measurement one can make is at the single molecule level; it just does not get any better than that. To determine the concentration of a molecule in solution, the best way is to count the number of molecules in a given by: The single molecule detection techniques are based on the two key techniques of single molecule imaging and single molecule manipulation.
Light microscopy is a method that allows us to image biomolecules as they work in an active manner in biomolecular assemblies.
Consequently, the essential components of a single molecule detection set-up are: pulsed excitation source. single photon sensitive detector.
dichroic mirror (to separate fluorescence signal from excitation light) objective (to focus the excitation light into the sample and collect fluorescence signal). Single Molecule Detection in the Near‐Infrared. Steven A. Soper. Department of Chemistry, Louisiana State University, Baton Rouge, LA ‐, USA Single Molecule Detection in the Near‐IR in Polymer Microfluidic Devices Citing Literature.
Single Molecule Detection in Solution: Methods and Applications. Related; Information Cited by: 3. Book chapterFull text access. Chapter 8 - Single Ion Channels Pages Publisher Summary This chapter focuses on the use of single molecule studies on single ion channels.
It first reviews results obtained with single molecule fluorescence methods and compares them with single ion channel results.
Counting molecules is the most accurate method for measuring the concentration of molecules in solution. As discussed in this Feature, optical methods for single molecule detection typically amplify the target molecule, as in the case of Cited by: The Nobel Prize in Chemistry recognizes the work of Eric Bertzig, Stefan W.
Hell, and William E. Moerner, who developed techniques in super-resolved fluorescence microscopy. One of the first breakthroughs in this field was the optical detection of a single molecule inside a solid crystal by Moerner in Multiparameter single molecule fluorescence detection with applications to FRET Single-particle tracking-photoactivated localization microscopy (sptPALM) within live cells Site-specific incoporation of fluorescent probes into RNA polymerase Quantitative single-molecule imaging by confocal laser scanning microscopy.
Key to successful single-molecule detection is the need to optimize signal-to-noise ratio, and the physical parameters affecting both signal and noise are described in detail. Four successful microscopic methods including the wide-field techniques of epifluorescence and total internal reflection, as well as confocal and near-field optical Cited by: This review describes various microwell array formats and explores their applications for the detection and investigation of single enzyme molecules.
The development of new fabrication techniques and sensitive detection methods drives the field of single molecule enzymology.Single molecule sensing with solid-state nanopores: novel materials, methods, and applications Benjamin N. Miles, † a Aleksandar P. Ivanov, † a Kerry A. Wilson, a Fatma Doğan, a Deanpen Japrung ab and Joshua B.
Edel * aCited by: